Topflash reporter system
WebUpon TOPFLASH analysis, Cby-induced suppression of reporter activity was augmented significantly in HEK293 cells as TC1-siRNA was increased to 10 nmol/L (P < 0.004, ANOVA test), showing the... Web17. okt 2024 · Luciferase activity was measured with ONE-Glo™ Luciferase Assay System (Promega) and normalized with FOPFlash samples. All experiments were performed at least three times and each experiment contained three technical replicates. ... d DLD1 cells with or without MYC overexpression were transfected with TOPFlash or FOPFlash luciferase …
Topflash reporter system
Did you know?
WebThe Dual-Luciferase® Reporter (DLR™) Assay System provides an efficient means of performing two reporter assays. In the DLR™ Assay, the activities of firefly ( Photinus pyralis) and Renilla ( Renilla reniformis or sea pansy) … WebNational Center for Biotechnology Information
WebThis is a luciferase reporter of beta-catenin-mediated transcriptional activation. In HEK cells, maximal activation of this reporter is ~100-fold (activation by Wnt) up to ~1,000-fold (activation by phosphorylation mutants of beta-catenin). The appropriate control plasmid is clone M51, Super8XFOPflash, which has mutant TCF/LEF binding sites. WebThe artificial TOP reporter system is a widely used tool in the investigation of Wnt/β-catenin signaling, including genomic and drug screens (42, 43). However, the relevance of this …
WebThe first transcription-based luciferase reporter of Wnt/β-catenin signaling, TOPFlash, was designed by Korinek et al. ( 4 ). The TOPFlash reporter contains three TCF response … WebArticle Snippet: TOPflash/FOPflash system consisting of optimized TCF binding sites (TOP) or mutated sites (FOP) controlling the expression of a luciferase reporter gene was from …
WebLuciferase reporter assays allow you to investigate promoter activity by measuring light output from luciferase enzyme that is expressed under the control of your promoter of interest. Luciferase genes vary in stability, light output and spectral characteristics, and reporter kits are available with different luciferases for both single- and dual-spectral …
WebSKU: LTV-0011. $ 595.00. Fluorescent TCF/LEF Reporter Lentivirus (Wnt/Beta-Catenin Pathway): High-quality lentiviral TOPFlash reporter system that provides fluorescent readout in response to transcriptional … pinotti sasWebBT-549 cells were transiently transfected with TOP-FLASH firefly luciferase reporter vector and Renilla luciferase vector. After 24 hour-transfection, cells were treated with DMSO or 25 μM iCRT-3 for 48 hours. Luciferase activities were … pinotti itajaiWeb( E ) TOPFlash reporter assay in human embryonic kidney (HEK) 293T cells treated with conditioned medium from WT, Bmal1 −/− , Apc +/− , and Apc +/− ; Bmal1 −/− organoids. Luciferase activity from each genotype is shown normalized to WT organoids, and RLU are shown ( n = 3 independent organoid lines per genotype). haile selassie ethiopia lion of judahWebTOPFlash/FOPFlash assays. TOPFlash is a luciferase reporter that contains a minimal fos promoter coupled to Tcf-binding sites upstream of a modified firefly luciferase gene. … pinotti saraWebThe Luciferase Assay System is an extremely sensitive and rapid reagent for quantitation of firefly luciferase. Linear results are seen over at least eight orders of magnitude of enzyme concentration, and less than 10 –20 moles of luciferase can be … pinotti outletWebThe Luciferase Reporter 1000 Assay System (Cat.#. E4550) was designed to meet the needs of users who perform a large number of assays, particularly in 96-well plates. The … haile selassie jamaica visitWeb22. aug 2024 · Insights into the underlying mechanisms of competitive endogenous RNAs (ceRNAs) were determined by bioinformatics analysis, dual-luciferase reporter assays, … pinotti silvia