Tg fabp10a:cfp-ntr
WebMethods We used transgenic zebrafish, Tg (fabp10a:CFP-NTR), to study the effects of compounds that alter epigenetic factors on BEC-mediated liver regeneration. We analyzed zebrafish with disruptions of the histone deacetylase 1 gene (hdac1) or exposed to MS-275 (an inhibitor of Hdac1, Hdac2, and Hdac3). WebMethods: Using Tg (fabp10a:CFP-NTR) zebrafish, we examined the effects of 44 selected compounds on BEC-driven liver regeneration. Liver size was assessed by fabp10a:DsRed …
Tg fabp10a:cfp-ntr
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WebFollowing pharmacogenetic ablation of hepatocytes in Tg(fabp10a:CFP-NTR) zebrafish, resulting in severe liver injury, signal transducer and activator of transcription 3 (Stat3) and its target gene and negative regulator, socs3a, were upregulated in regenerating livers. WebTo ablate hepatocytes, Tg(fabp10a:CFP-NTR) larvae were treated with 10 mmol/L metronidazole (Mtz) in egg water supplemented with 0.2% DMSO and 0.2 mmol/L …
Web1 Mar 2014 · Methods. Hepatocytes were depleted by administration of metronidazole to Tg(fabp10a:CFP-NTR) animals. We traced the origin of regenerating hepatocytes using … Web1 Feb 2016 · Methods. Using Tg(fabp10a:CFP-NTR) zebrafish, we examined the effects of 44 selected compounds on BEC-driven liver regeneration. Liver size was assessed by …
WebThe Tg(fabp10:dsRed, ela3l:GFP) gz12 transgenic line was generated with the fluorescent protein DsRed and Tg(fabp10a:CFP-NTR) gt1 was generated with the fluorescent protein Cyan fused to NTR and driven by the hepatocyte-specific promoter lfabp. 31 The transgenic line was adopted to confirm the PG effect of liver regeneration on antibiotics. The adult … WebApproach results: Towards this aim, we have established a zebrafish model for hepatocyte-to-BEC conversion by employing Tg(fabp10a:CFP-NTR) zebrafish with a temporal …
WebTg(fabp10a:CFP-NTR) [27–29] Mouse CDE diet Chemical BEC-driven regeneration Ethionine, a toxic analog of methionine, in association with choline deficiency, leads to hepatocyte death and liver inflammation [30] Ctnnb1 hepatocyte KO Genetic BEC-driven regeneration
WebGeneration of the Tg(fabp10a:rtTA,TRE:Venus-KRAS) line . Gateway cloning (Thermo fisher Scientific) was used to make the fabp10a:rtTA-pA,TRE:CFP-pA construct with three entry … peter\\u0027s pharmacy three hillsWeb4 Jul 2014 · Similar to that observed in the larvae, the CFP and dim mCherry coexpressing cells were detected both in the MTZ- and EtOH/MTZ-treated regenerating livers of 12-month-old [Tg(fabp10a:CFP-NTR) gt1; Tg(Tp1:mCherry) jh11] adult fish at 3 days-post-ablation (dpa) (Fig. 2E″,F″, insets with arrows) with markedly elevated deposition of type I collagen … startengine how it worksWebthe hepatocytes of Tg(fabp10a:CFP-NTR) larvae and blocked Notch activation during liver regeneration through treatment with γ-secretase inhibitor LY411575 and demonstrated enhanced induction of Hnf4a in LPCs. Alternatively, enhancing Notch signaling via Notch3 intracellular domain (N3ICD) overexpression impaired Hnf4a induction. Hepatocyte ... peter\u0027s pies caerphillyWebUsing Tg(fabp10a:CFP-NTR) zebrafish, we examined the effects of 44 selected compounds on BEC-driven liver regeneration. Liver size was assessed by fabp10a:DsRed expression; liver marker expression was analyzed by immunostaining, in situ hybridization and quantitative PCR. Proliferation and apoptosis were also examined. Moreover, we used a … start engine in cold weatherWeb1 Jan 2024 · Tg(fabp10a:CFP-NTR);Tg(fabp10a:DsRed) adult fish. Mating tanks: large aquaculture tanks with dividers. Fine plastic mesh strainer (tea strainer). Squeeze bottle. … startengine investor dashboardWebThat Utilize Tg2(fabp10a:CFP-NTR) Construct Fish Affected Genomic Regions Phenotype Gene Expression; gt1Tg: gt1Tg; gz15Tg: Fig. S1 from Huang et al., 2014 Fig. S1 from … startengine knightscopeWeb14 Aug 2024 · In Tg(fabp10a:pt-β-catenin) zebrafish larvae, ... Our group developed the Tg(fabp10a:CFP-NTR) zebrafish line that expresses nitroreductase (NTR) under the … start engine investment account