Hct116 puromycin
Web嘌呤霉素（Puromycin）是一种由白黑链霉菌产生的氨基苷类抗生素。其可以打乱核糖体上的肽转运，造成翻译过程中不成熟链终止，从而抑制蛋白质合成。在原核和真核细胞 … WebApr 30, 2024 · To obtain cell lines stably overexpressing the lncRNA Ftx, HCT116 cells were transfected with Ftx and screened with puromycin (2 μg/mL), whereas HCT116 cells transfected with Ftx-NC were used as its mock control. The stable lncRNA Ftx-interfered or -overexpressing cell lines were validated by real-time PCR with the following primers …
Hct116 puromycin
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http://cell-lines.toku-e.com/Cell-Lines_1422.html WebJan 10, 2024 · Briefly, growth media containing DMEM (high glucose, pyruvate, ThermoFisher Scientific, Waltham, MA, USA) for HeLa Kyoto and HCT116 cells and …
WebFind jobs, housing, goods and services, events, and connections to your local community in and around Atlanta, GA on Craigslist classifieds. WebApr 1, 2024 · After 72 h of transfection, 2 mg/ml puromycin was added to the culture medium for HCT116 or RKO selection, respectively. ... A HCT116 cells in the siHOXC6 group were more sensitive to 5-Fu, ...
WebHCT 116 Cell Line. HCT116 is a human colorectal carcinoma cell line initiated from an adult male. The cells are adherent with an epithelial morphology. Following implantation into … WebMar 25, 2024 · Next, we constructed knockout plasmid by cloning the sgRNA into the pCasCMV-Puro-U6 vector. After transfection of the plasmid into HCT-116 cells, TrxR1 knockout HCT-116 cells were selected using puromycin resistance. The TrxR1 knockout efficiency was identified and verified by DNA sequencing, immunoblotting, TRFS-green …
WebMar 3, 2024 · Deletion of the BH3 domain of Bax reduced the complexes formed with the K21E and D33A mutants in HeLa and HCT116 Bax −/− cells (Figure 8 g,h). Taken together, these results suggest that the α1 helix could act as a binding region for Bax-BH3 domains but would not be critically involved in the assembly of dimers through the rear surface.
WebProtocol: Puromycin, Blasticidin and Hygromycin Titration Brief Description: A lentiviral construct carrying an antibiotic resistance gene will allow selection of the cell population … foods ebase cloudWebApr 7, 2024 · In line with these, we observed protein levels of DDX21 in most of CRC cell lines, such as HCT116, LOVO and RKO, are significantly higher than those in normal colonic epithelial cell NCM460 except ... electrical engineer jokesWebHCT116 cells have been widely used in colorectal cancer research. HCT116/GFP (CMV) stable cells express GFP under the control of the CMV promoter. ... Puromycin: Biosafety Level: BSL-2: Shipped: Dry ice : Storage: Liquid Nitrogen: Catalog Number Product Size Price Qty; SC-1001. HCT 116/GFP (CMV) stable cells. 2x10^6 cells: Price: Quantity. … electrical engineer mcatWebGeneticin (G418 sulfate), zeocin, hygromycin B, puromycin, and blasticidin antibiotics are the most common selection antibiotics used for stable cell transfection. These antibiotics provide unique solutions for your research needs, such as dual selection and rapid, stable cell line establishment. Explore all Gibco selection antibiotics electrical engineer level 5 salaryWebApr 24, 2024 · Notably, puromycin upregulated the expression of RPL5 and RPL11 to directly bind to MDM2 in HCT116 cells. Conversely, deletion of RPL5 and RPL11 … electrical engineer mba salaryWebSep 16, 2024 · To examine the role of CTRP1 in tumor progression, we generated CTRP1 knockout A549 and HCT116 cell lines, which reduced the expression levels of nuclear factor (NF)-κB-dependent and metastasis-promoting transcripts. ... and cells were selected with puromycin (2 µg/mL). Control cells were generated by transduction of an empty vector in ... food secretary sudhanshu pandeyWebMar 14, 2024 · Puromycin was bought from Sigma-Aldrich (St. Louis, MO, USA). Firefly luciferase-lentiviruses and Polybrene were obtained from GENECHEM (Shanghai, China). D-luciferin potassium salt was obtained from PROMEGA (Madison, WI, USA). ... Compared to HCT116 and SW620 tumor-bearing mice, other groups (SW480, HT29, LOVO and … electrical engineer max salary