WebMay 5, 2024 · In vitro maturation of human primary myoblasts using 2D culture remains a challenging process and leads to immature fibers with poor internal organization and function. This would however represent a valuable system to study muscle physiology or pathophysiology from patient myoblasts, at a single-cell level. Human primary myoblasts … WebMar 16, 2024 · In order to experimentally evaluate myoblast fusion in vitro, a fusion index is calculated based on detection of immunofluorescence by microscopy. Fluorescently labeled antibodies are used to detect muscle fiber structural proteins such as myosin … ŽTÔ•!D ÑŸÀ ÊЛ3f §¶;™‚HŠ]oú²=Åcu˜Š`úD„ ŠêN%A ¶=¶ê … BioTechniques is a MEDLINE-indexed, peer-reviewed, open access journal that … Digital Features in our Journals. All our journals can feature a range of digital … BioTechniques is a MEDLINE-indexed, peer-reviewed, open access journal that …
Analysis and quantification of in vitro myoblast fusion using the LADD …
WebJun 22, 2024 · In 2D, enhanced myogenesis was observed, as evidenced by an increased myotube diameter and fusion index. Second, 2D results were translated towards 3D … WebFeb 14, 2024 · ICC allows for quantification of the fusion index, which is defined as the percentage of myoblast nuclei that have fused into fibers in a single time point . Fusion indices are the main tool for assessment of the differentiation potential of myoblasts . Newer live cell imaging tools allow assessment of myoblast differentiation in real-time. theater in hastings mi
Analysis and quantification of in vitro myoblast fusion …
WebDec 1, 2016 · Myoblast fusion, which is essential for muscle development, regeneration, and repair, can be assessed in vitro via the calculation of a fusion index. Traditionally, … WebOct 19, 2024 · The fusion index of wild-type cultures was ∼58% after 7 days of differentiation, with an average of 6.9±2.1 nuclei per myotube (mean±s.d.; ... Taken together, our findings indicate that the regulation of myoblast fusion is more complex than previously anticipated and may involve both CDC50A-dependent (including different … WebMay 11, 2024 · The myoblast fusion index was calculated as the ratio of the number of nuclei inside myotubes compared to the total number of nuclei × 100 at day 10 of myogenic differentiation via ImageJ software. Co-localization was quantified with the Coloc2 plugin for the ImageJ software program ... theater in highland park