WebTHPTA is a water-soluble, very effective ligand for Cu(I)-catalyzed Alkyne-Azide click chemistry reactions (CuAAC). It serves a dual purpose: 1) Acceleration of the CuAAC reaction by maintaining the Cu(I) oxidation state of copper sources and 2) Protection of biomolecules from oxidative damage during the labeling reaction. THPTA is a superior … WebBTTAA has been found to be the most efficient that allowed the use of cop-per(I) catalyst at a concentration that does ... 19. Jewett JC, et al. Rapid Cu-free click chemistry with readily synthesized biarylazacyclooctynones. J Am Chem Soc. 2010;132(11):3688–3690. 20. Besanceney-Webler C, et al. Increasing the effi-
CuAAC Biomolecule Reaction Buffer Kit (BTTAA based) - Jena …
WebApr 14, 2024 · DBCO Agarose Cat.# 1034-2 / Size: 2ml Cat.# 1034-25 / Size: 25ml DBCO agarose is a 6% crosslinked agarose resin that is activated with DBCO functional groups for covalent capturing azide-tagged biomolecules utilizing Cu-free click reaction. This product is adequate to work in batch or column purification (low pressure). Specification Enrichment … WebThe CuAAC Biomolecule Reaction Buffer Kit (BTTAA based) provides sufficient amounts to perform 25 CuAAC experiments à 200 μl using 2 mM CuSO 4, 10 mM BTTAA and 100 mM Na-Ascorbate in 100 mM Na-Phosphate reaction buffer. A general protocol for labeling of biomolecules (see 3.) is outlined below. practice writing free printable
BTTAA Cu(I)-stabilizing Ligand MedChemExpress
WebClick Chemistry Azido-, alkyne-, and DBCO containing biotinylation reagents for bioconjugation, biolabeling and chemical modification. Biotin can be subsequently … WebDec 18, 2024 · Click Reaction Cocktail Dissolve 4.44 mg azide- (PEG) 3 -biotin to 1 mL DMSO (C f = 10 mM). Aliquot the stock to 50 μL and store at −20°C. Dissolve 1.25 mg copper sulfate pentahydrate to 100 μL of water and 4.3 mg BTTAA to 200 μL of water. WebBTTAA is a next-generation, water-soluble ligand for the copper (I)-catalyzed azide-alkyne cycloaddition (CuAAC) that dramatically accelerates reaction rates and suppresses cell cytotoxicity. schwan\u0027s ham and cheese omelet